Molecular Marker Technology
📍Morphological markers
📍Biochemical Markers
📍Genetic markers.
↪️Dominant and co-dominant nature of
markers with their specific applications
↪️Study of different markers systems with their limitations and strengths
📍RFLP
📍PCR based markers
🔹RAPD
🔹SCAR
🔹SSR
🔹STS
🔹CAPS
🔹AFLP, SNP
🔹and their variants.
↪️Uses of molecular markers
📍Applications of molecular markers in various fields with special reference to crop improvement
📍Application as a genetic tool for genotyping and gene mapping.
↪️Mapping populations
📍Different types of mapping populations
🔹F2
🔹DH
🔹RILs
🔹NILs.
📍Methods of development and segregation
pattern in each type of population
📍Merits and demerits of each population.
📍Evaluation criteria for each type of
population.
📍Application of mapping population in molecular marker studies
↪️Bulked segregant analysis
📍Analysis technique used for Bulked
segregant analysis
📍Applications of Bulked segregant analysis
↪️Linkage maps & Physical maps
📍Construction of linkage map and physical map
📍Application of Linkage maps and Physical maps
↪️Application of molecular markers
📍Assessing genetic diversity, variety protection
↪️Application of molecular markers
📍Marker-assisted breeding for accelerated introgression of trait/transgene
📍Marker-assisted breeding for quantitative traits
↪️Application of molecular markers
📍Human and animal health: Association with genetic-based diseases:
📍Genetic variations in the human genome
📍Genetic testing for genetic-based diseases
↪️Application of molecular markers
📍Paternity determinations
🔹Determinations of paternity by molecular genetic "fingerprinting".
📍Advantages of DNA fingerprints
🔹For determination of correct paternity
🔹For gentic linkage and pedigree studies
↪️Application of molecular markers
📍Forensic studies: Applications, implications
↪️Limitations of Molecular forensics
@Syllabus mpkv rahuri
No comments